Software for TCSPC
We offer a range of software packages to complement your TCSPC systems.
Packages are available for:
- Instrument control and visualization (SPCM)
- FLIM Data Analysis (SPCImage)
- Single Molecule Burst Analysis (Burst Analyzer)
SPCM data acquisition software is provided free with all Becker & Hickl TCSPC modules and DPC-230 photon correlators. SPCM is 64-bit software for Windows for megapixel FLIM technology:
- Acquisition of FLIM images
- Online calculation and fit of FCS curves
- Online calculation of PCH, and MCS traces
- Configurable for different instrument configurations
- Easy change between different configurations
- Parallel operation of up to four SPC or DPC modules
- Online display of images and curves
- Full access to all functions of the boards
- One software for all bh TCSPC modules
- Page stepping
- Accumulation and autosave functions
- Oscilloscope mode
- Single and repeat mode
- Sequential modes
- Imaging modes
- Time tag mode
- Time tag imaging mode
- Integrated scanner control
- Interaction of scanner control with display cursors
- 2D and 3D display modes
- 3D Curve mode, colour-intensity mode
- Software gating of image display
- Display of multi-wavelength FLIM images
- 2D and 3D data processing functions
- Loading of predefined setups
- Saving, loading, and conversion of photon distributions,images, and time-tag data
SPCImage – NG (Next Generation) Analysis Software for FLIM, PLIM, Fluorescence and Phosphorescence Decay
- Combination of Time-Domain and Phasor Analysis
- Maximum-Likelihood Fit
- GPU Processing
- Calculation of FLIM Images within less than 1 second
- Single, Double, Triple-Exponential Models
- Incomplete-Decay Models
- Analysis of Single Decay Curves, FLIM Data and PLIM Data
- Calculation and Display of Lifetimes, Amplitudes, Fractional Intensities
- Calculation and Display of Ratios of Decay Parameters
- Calculation and Display of FRET Efficiencies
- One- and Two-Dimensional Histograms of Decay Parameters
- Batch Processing
- Export of Lifetime Data and Images
- Calculation of FCS and cross-FCS
- FCS fit with user-defined model functions
- Identificaction of single-molecule photon bursts in parameter-tag data
- Analysis of fluorescence intensity within photon bursts
- Analysis of fluorescence lifetime within photon bursts
- One- and two-dimensional histograms of burst parameters
- Filtered histograms of burst parameters
- Discrimination of different fluorescent species
- Determination of FRET efficiencies
- Discrimination of different FRET states
- Exclusion of artefacts in intensity traces
LabVIEW libraries are offered for the following
for the following modules:
- SPC-13x
- SPC-140
- SPC-15x
- SPC-16x
- SPC-7×0
- SPC-6×0
- SPC-830
- DPC-230
DLL libraries for programming in e.g. C++ are available for the following modules:
- SPC-13x
- SPC-140
- SPC-15x
- SPC-16x
- SPC-7×0
- SPC-6×0
- SPC-830
- DPC-230
