Ultra-Fast Fluorescence Lifetime Imaging Module

The -NXX version of the SPC-180 time correlated single-photon counting (TCSPC) FLIM module from Becker & Hickl GmbH features ultrahigh time resolution and timing stability. The internal instrument response function (IRF) is 2.8-ps full width half maximum, or 1.1 ps RMS. IRF stability is >0.4 ps over 100 s. Decay data can be resolved into 4096 time channels, with a minimum channel width of 202 fs. The module offers the usual modes for recording temporal waveforms of optical signals, sequential recording, time- and parameter-tag recording, FLIM, spatial and temporal mosaic FLIM, triggered accumulation of fast time series of curves and images, and simultaneous FLIM/PLIM (phosphorescence lifetime imaging). Pixel numbers in FLIM mode can be as high as 4096 × 4096, and pixel rates can be in the megahertz range. A high-speed PCI Express interface provides high data throughput at high photon and pixel rates.